mouse lif  (MedChemExpress)

Journal: bioRxiv

Article Title: Resolving heterogeneity of targeted lipid nanoparticles through solution-based biophysical analyses

doi: 10.64898/2026.03.31.715590

Figure Lengend Snippet: ( a-b ) NT LNPs and tLNPs containing FLuc mRNA were administered intravenously via retroorbital injection into pregnant and nonpregnant mice at a dose of 12 µg mRNA per mouse. After 6 h, mice were euthanized, and serum was collected. Serum levels of C3a, TNF, IFN-γ, IL-6, ALT, and AST were quantified in ( a ) pregnant and ( b ) nonpregnant mice via ELISA. Measurements are reported mean ± SD from n = 3–4 biological replicates. One-way ANOVA with post hoc Student’s t-tests using the Holm–Sídak correction for multiple comparisons was used to compare cytokine levels across treatment groups. ( c–e ) Spearman correlations for ( c ) TNF, ( d ) IFN-γ, and ( e ) IL-6 serum levels in pregnant (top) and nonpregnant (bottom) mice using the physicochemical parameters from traditional characterization methods, static SAXS analyses, and AF4-UV-DLS-MALS-SAXS analyses. ( f ) Heatmap representing the entire dataset. For Spearman correlation graphs, dotted lines represent r = –0.6 and 0.6.

Article Snippet: Mouse Quantikine ELISA kits (R&D systems) were used to evaluate C3a, IL-6, TNF, and IFN-γ levels in serum per the manufacturer’s instructions.

Techniques: Injection, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: The LIF-LIFR Axis Promotes Liver Regeneration via Modulation of Angiogenesis and HGF Release from LSECs

doi: 10.64898/2026.02.24.707802

Figure Lengend Snippet: (A) lif expression in the scRNA-seq from mouse livers with acetaminophen (APAP)-induced injury at the indicated time points. (B) lif expression in the scRNA-seq across cell types in the livers of mouse with APAP injury at the indicated time points. (C) lif expression in spatial transcriptomics of liver samples from healthy (HEA2) and APAP injury (APAP1_S2) humans. (D&E) Serum mLIF levels at 24, 48, and 72 h after partial hepatectomy (PHx) in mice treated with IgG (D) or anti-LIF neutralizing antibody (E). (F) Resected liver-to-body weight ratio in mice treated with anti-LIF antibody or control IgG. (G) Liver-to-body weight ratio at 24, 48, 72 h and 1-week post-PHx in mice treated with anti-LIF antibody or control IgG. (H) Immunofluorescence staining of liver sections at 48 h post-PHx from mice treated with anti-LIF antibody or control IgG. DAPI (blue), PCNA (red), CK19 (purple). Scale bars: 100 μm. (I) Quantification of PCNA-positive hepatocytes ratio at 48 h post-PHx in mice treated with anti-LIF antibody or control IgG.

Article Snippet: The following steps were carried out according to the manufacturer’s protocols from mLIF ELISA kit (R&D system, MLF00).

Techniques: Expressing, Spatial Transcriptomics, Control, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: The LIF-LIFR Axis Promotes Liver Regeneration via Modulation of Angiogenesis and HGF Release from LSECs

doi: 10.64898/2026.02.24.707802

Figure Lengend Snippet: (A-C) Body weight (A), liver weight (B) and liver-to-body weight ratio (C) of mice injected with AAV8-TBG-mLIF at the indicated titers. (D) Serum mLIF levels in mice injected with AAV8-TBG-mLIF at the indicated titers. (E&F) CD31 immunofluorescence staining of liver sections from mice injected with low-doses AAV8-TBG-mLIF (1×10⁸ or 2.5×10⁸ GC/mouse) (E) and quantified in (F). Scale bar=50 μm. (G) Serum HGF levels in mice injected with AAV8-TBG-mLIF at the indicated titers. (H) Liver-to-body weight ratio at the indicated time points post-PHx in mice injected with low-doses AAV8-TBG-mLIF (1×10⁸ or 2.5×10⁸ GC/mouse). (I&J) Serum mLIF (H) and HGF (I) levels at 24, 48 and 72 h post-PHx in mice injected with low-doses AAV8-TBG-mLIF (1×10⁸ or 2.5×10⁸ GC/mouse).

Article Snippet: The following steps were carried out according to the manufacturer’s protocols from mLIF ELISA kit (R&D system, MLF00).

Techniques: Injection, Immunofluorescence, Staining